骨关节炎
软骨
间充质干细胞
脂肪组织
医学
移植
病理
内侧副韧带
干细胞
韧带
解剖
外科
内科学
生物
细胞生物学
替代医学
作者
Qianqian Wang,Shibo Su,Chuanming Dong,Qi Fan,Jingwei Sun,Shenzhi Liang,Zailong Qin,Congqian Ma,Jianfeng Jin,Hongwen Zhu,Tongmeng Jiang,Jian Xu
出处
期刊:Current stem cell research & therapy
[Bentham Science]
日期:2024-01-08
卷期号:19
标识
DOI:10.2174/011574888x274359231122064109
摘要
Objective:: In recent times, it has been recognized that mesenchymal stem cells (MSCs) possess the capability to address osteoarthritis (OA). The objective of this research was to examine the impact of injecting human adipose-derived stem cells (hADSCs) into a novel rabbit osteoarthritis model with dual damage. Methods:: The OA model was established surgically first by medial collateral ligament and anterior insertional ligament transection and medical meniscectomy, then by articular cartilage full- thickness defect. Enhanced Green Fluorescence Protein expressing lentivirus FG12 was used to label hADSCs, which were then injected into the knee joints. Every single rabbit was sacrificed after 4 and 8 weeks following the surgical procedure. Macroscopic examination, immunohistochemistry staining, magnetic resonance imaging, qRT-PCR, and ELISA analysis were utilized for the assessments. Results:: After 4 and 8 weeks, the injection of hADSCs resulted in reduced cartilage loss, minimal fissures and cracks, and a decrease in the volume of joint effusion and cartilage defect as measured by MRI. Moreover, the application of ELISA and qRT-PCR techniques revealed that the administration of hADSCs resulted in an elevation in the IGF-1 concentration. Conclusions:: Based on our findings, it can be inferred that the transplantation of hADSCs facilitates the healing of articular cartilage in the osteoarthritis model of rabbits with double damage. The upregulated IGF-1 may play a crucial part in the process of cartilage repair using hADSCs. The use of hADSC transplantation could potentially be appropriate for clinical implementation in managing osteoarthritis.
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