睾丸决定因素
生物
染色质免疫沉淀
基因敲除
下调和上调
异位表达
转录因子
染色质
分子生物学
报告基因
免疫沉淀
癌症研究
基因表达
基因
发起人
遗传学
Y染色体
作者
Shigekazu Murakami,S Chishima,Hiroyoshi IsodaShinji Uemoto,Erina Sakamoto,Tsugumichi Sato,Nobuya Kurabe,Yasushi Kawasaki,Tatsuhiro Shibata,Hirotada Akiyama,Fumio Tashiro
出处
期刊:Oncogene
[Springer Nature]
日期:2013-07-29
卷期号:33 (23): 2978-2986
被引量:26
摘要
Sgf29, a component of the SPT-ADA-GCN5 acetyltransferase (SAGA) complex, binds H3K4me2/3 marks and leads to histone H3 acetylation. Previously, we found that downregulation of Sgf29 suppresses c-Myc-mediated malignant transformation. Nonetheless, the upstream regulator of the Sgf29 gene is not yet known. Here, we report that Sry (sex-determining region Y), an HMG (high-mobility group) domain containing transcription factor, directly upregulates Sgf29 gene expression. Sry expression was deregulated in two out of the four tested male rodent hepatocellular carcinoma (rHCC) cell lines. Luciferase reporter and chromatin immunoprecipitation assays indicated that Sry could bind HMG-boxes in the proximal promoter region of the Sgf29 gene. Knockdown of Sry robustly lowered anchorage-independent growth, invasiveness and tumorigenicity of rHCC cells, whereas ectopic expression of Sry conferred more malignant properties. Thus, these data show that Sry is involved in male-specific malignant conversion of rHCCs via Sgf29 upregulation.
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