硝苯地平
化学
色谱法
代谢物
萃取(化学)
高效液相色谱法
全血
定量分析(化学)
血浆
生物化学
内科学
钙
医学
有机化学
作者
P. A. Soons,J.H.M. Schellens,M.C.M. Roosemalen,D.D. Breimer
标识
DOI:10.1016/0731-7085(91)80249-9
摘要
A reversed-phase HPLC method is described for the simultaneous determination of nifedipine and its primary pyridine metabolite dehydronifedipine in blood and plasma, that involves UV detection and neutral (blood) or alkaline (plasma) extraction. The limit of reliable determination is found to be 3 ng ml-1 with an inter-assay RSD of below 11%. In the presence of haemoglobin, nifedipine is unstable at pH greater than 10, necessitating neutral extraction for the measurement of nifedipine in haemolysed blood. Published methods for analysis of nifedipine are reviewed, emphasizing the lack of specificity and sensitivity which render many of them unsuitable for the investigation of nifedipine disposition in man.
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