脑脊液
烯醇化酶
病理
脑炎
医学
脑膜脑炎
脊髓
免疫组织化学
免疫学
病毒
精神科
作者
Kozo Nakamura,Taku Miyasho,Sadahiro Nomura,Hiroshi Yokota,Tetsuya Nakade
出处
期刊:Journal of Veterinary Medical Science
[Japanese Society of Veterinary Science]
日期:2012-01-01
卷期号:74 (6): 751-756
被引量:22
摘要
We performed proteomics analysis of the cerebrospinal fluid (CSF) of healthy dogs and dogs with meningoencephalitis of unknown etiology (MUE). By comparing two-dimensional electrophoreses (2DE), an upregulated spot was found in MUE dogs. This protein was identified as a neuron-specific enolase (NSE) by analysis with MALDI-TOF mass spectrometry. In comparing dot blots using an antibody against NSE, the NSE levels in the CSF of MUE dogs was significantly higher than that of the controls. NSE is a diagnostic marker of neuroendocrine tumors, brain injury and spinal cord trauma in humans. It seems that the NSE concentration in the CSF is increased by cellular destruction in canine encephalitis. Though elevation of NSE may not be specific in canine encephalitis because the NSE level was increased in other CNS diseases, further study including measurement with serum is necessary.
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