Abstract bis‐Phosphatidic acid from developing soybean was completely hydrolyzed with phospholipase A2 (lyophilized venom from Ophiophagus hannah) to yield lyso‐bis‐phosphatidic acid and free fatty acids. It is suggested that soybean bis‐phosphatidic acid is derived from sn‐3‐glycerophospho‐sn‐3′‐glycerol.