A study on vaginal dysbacteriosis caused by antibiotics and the adjustment by Lactobacillus.

Objective To produce the mouse models of vaginal microflora dysbiosis induced by antibiotic,and study the adjustment of Lactobacillus on vaginal infection.Method 50 mg/d ceftriaxone was given to the IRC mice intragastrically for 5 days and inoculated intra-vaginally with 20 μl of a suspension of Candida albicans to make mouse vaginal candidias albican imbalance model.The model mice were then inoculated intra-vaginally with 20 μl of a suspension of 2×108CFU/ml Lactobacillus.After treatment the Candida albicans and Lactobacillus were quantified by culture of vaginal lavage fluid and Papanicolaou staining of vaginal tissue.Result The amounts of Lactobacillus in the vaginal of model group decreased significantly(P0.01).White blood cells and Candida albicans were found through vaginal secretion examination by microscopy.After treatment,the amount of Lactobacillus in the vagina of the mice treated by Lactobacillus increased and recovered to normal level(P0.05),while the amount of Candida albicans decreased significantly.We also found that the rebuilt viginal microbiota could exclude Candida albicans dramatically,which indicated that the colonization resistance of the flora was recovered(P0.01).Interestingly,all of the efficiency was impressed with the Lactobacilus pre-treated group.Conclusion The adhesion of Candidias albican to vaginal mucous membrane could be prevented by Lactobacilus imported from outside the vagina.The vaginal microecosystem could be adjusted and the vaginal mucous membrane could be mended by Lactobacilus.