Objective To understand and analyze the malaria situation in Yongcheng City in 2007 and to evaluate different malaria prevention and control measures. Methods Data on the malaria epidemic situation were collected and analyzed. Malaria antibody levels and vector were evaluated by IFAT and vector surveys, respectively, and different malaria prevention and control measures were evaluated. Results The malaria incidence in 2007 was 0.16, which was a 45.02% decrease from that of 2006. The malaria antibody positive rate and GMRT in 2007 were greater than in 2006. Anopheles sinensis was the only vector in Yoncheng City, and a vector survey showed that the man biting rate, multifarious rate and human blood index were 3.75/person, 54.55% and 0.666 7, respectively. The vectorial capacity and basic reproductive rates were 0.689 and 1.453 6, respectively. The malaria incidence between different malaria prevention and control measures were not statistically different. The malaria incidence in villages with biologic control measures in 2007 dropped 51.0% and was statistically significant (χ2=118.099, P0.05). Conclusion The resurgence of malaria appeared in Yongcheng County, and malaria prevention and control measures suitable for the local situation will control the malaria situation effectively.