Study on determination of tocopherol in corn oil by HPLC

To establish two kinds of HPLC methods(normal phase HPLC chromatography and reversed phase HPLC chromatography) for determination of tocopherol in corn oil.To compare the difference between two kinds of HPLC methods.By normal phase HPLC chromatography,the column was silicagel column,the mobile phase was hexane:isopropanol=98∶2.The flow rate was 1.0 ml/min,the detective wavelength was 298 nm.The average recovery was 97.1% with RSD 2.00%.By reversed phase HPLC chromatography,the column was C18 column,the mobile phase was methanol: water=90∶10.The flow rate was 0.8 ml/min,the detective wavelength was 298 nm.The average recovery was 84.9% with RSD 2.29%.Compared with reversed phase HPLC chromatography,normal phase HPLC chromatography was more effective,stable and low cost.