cDNA Cloning,Genomic Structure of PM/mPM Gene from B.mori

[Objective] The experiment aimed to clone paramyosin(PM) and mini-paramyosin(mPM) genes to analyze the relations between them and moving behaviors.[Method] PCR method and RACE technology were used to obtain whole cDNA of PM and some cDNA of mPM of Bombyx mori.By compared wgs of Bombyx mori,the genomic sequences of PM and mPM of Bombyx mori were obtained,besides,their genome structure was determined.[Result] PM and mPM genes were consisted of 17 exons and 16 introns.By the use of selective promoters,the gene sequence encoded PM and mPM,while PM and mPM shared the last 6 exons.The cluster analysis between PM of Bombyx mori and PM of other invertebrates demonstrated that the relation between Bombyx mori and Bombyx mandarina was closest and the relation between Bombyx mori and Drosophila melanogaster was farthest in Insecta.[Conclusion] There was no point mutation which could influenced flight in amino acid sequence of PM of Bombyx mori and Bombyx mandarina,so the difference of flight capacity of Bombyx mori and Bombyx mandarina might be regulated by other mechanism.