Effects of salinity on the growth of Phaeodactylum tricornutum and content of organics in the P.tricornutum cells were investigated.And the culture condition of high yield of algal oil was also optimized.Results showed remarkably effects of salinities on biomass and accumulation of organics.The algal growth rate under 70‰ salinity was 8.2% lower than that of normal salinity(35‰).The highest biomass(dry weight 1.92g/L) and total lipid(29.98%) were produced under normal seawater cultures.The highest volumetric productivity of total lipid under 35‰ salinity was significantly different,with the algal oil productivity of 47.67mg per liter each day.Polysaccharides content of dry weight reached to the top of 5.5% under 17‰ salinity,and protein reached to the top of 17.82% under 70‰ salinity.