Comparison of 6,6-D_2 glucose and 3-~3H labelled glucose as a tracer in insulin clamp

objective To compare the differences between 6,6-D 2 glucose and 3- 3H labelled glucose as a tracer in evaluating glucose metabolism during extended hyperinsulinemic euglycemic clamp. Methords Twenty male Sprague-Dawley rats were divided into two groups, and an extended hyperinsulinemic-euglycemic clamp technique with 6,6-D 2 glucose or 3- 3H labelled glucose as a tracer was used.Glucose infusion rate(GIR), glucose disappearance rate(GRd) and hepatic glucose production (HGP) were evaluated.Results There were no significant differences in blood glucose,plasma insulin and FFA levels during the clamp between two groups, and also no significant differences in GIR, GRd and HGP. Conclusion 6,6-D 2 glucose and 3- 3H labelled glucose as tracers are consistant in evaluating glucose metabolism, but 6,6-D 2 glucose excludes the risk of radioactive contamination.