Effects of feeding broad bean on muscle texture characteristics,lipid accumulation and tissue fatty acid composition of grass carp(Ctenopharynodon idellus)

[Objective]The effects of feeding broad bean on biological character,fat accumulation,blood lipid content,fat acid compositions of grass carp were studied,in order to reveal mechanism of nutrition metabolism or lipid metabolism of crisp grass carp.[Method]A total of 32 grass carp samples were selected and divided averagely into two groups.These two groups were fed on broad beans and commercial compound feed,respectively,which were coded as CGC and GC,respectively.After these samples were dissected,their growth traits,biological characteristics,conventional ingredients,serum physiological indexes,dorsal muscle texture,and fatty acid composition were determined.[Result]The results showed that,the hepatosomatic index(HI),intraperitoneal fat ratio(IFR),body length to height ratio(LHR)and viscerossomatic index(VI) of CGC were extremely significantly higher than those of GC(P0.01,the same below),and the moisture content in muscles of CGC were significantly lower than those of GC(P0.05,the same below).Besides,the fat content of hepatopancreas significantly increased,but other conventional ingredients of hepatopancreas had no significant change(P0.05,the same below).The determined results of dorsal muscle texture showed that,dorsal muscle hardness,adhesiveness,chewiness and restoring force of CGC were increased by 31.1%,52.3%,47.8%and 56.3%,respectively,compared with those of GC.The meat quality evaluation results showed that the succulence and tenderness of CGC were lower than those of GC,and there was no significant difference in umami and flavor between CGC and GC.As for serum physiological indexes,only AST level(215.37±7.74 U/L) and TG content(3.73±0.35 mmol/L) of CGC were extremely significantly higher than those of GC,but there was no significant difference in other serum physiological indexes between CGC and GC.As for fatty acid composition,MUFA contents in muscle,hepatopancreas and abdominal fat of CGC were extremely significantly higher than those of GC,but the contents in SFA,n-6 PUFA and n-3 PUFA of CGC were extremely significantly lower than those of GC.[Conclusion]Lipid metabolism disorders is an important factor leading to slow growth of grass carp fed on broad bean.Therefore,in order to improve economic efficiency of crisp grass carp in aquaculture and feed development,the lipid metabolic pathways should be regulated,and growth rate should be improved without affecting muscle quality of crisp grass carp.