Study on Hydrolysis of Glycinin

According to Nagano method,glycinin was isolated from soybean cake and was hydrolyzed by soy protein modification enzyme to produce hydrolysate peptide.Optimum enzymatic hydrolysis conditions were determined by single-factor and orthogonal test.Then the distribution of glycinin hydrolysate peptide was detected by means of HPLC.Results showed that these optimum conditions were followed: 10 000 U/g of enzyme concentration,55 ℃,pH 8.0 and 4 h of reaction time when substrate concentration was 20 g/L,and the degree of hydrolysis(DH) was 69.6 % in these conditions.Glycinin hydrolysate peptides of 130 u-1 000 u occupied 86.5 % in total peptides,which showed that the peptide possessed excellent uniformity.