Determination of polysaccharide content in extracellular polymers of leaching bacteria

The leaching bacteria liquid in the stationary phase was studied.The optimum condition was discussed for the polysaccharide content determined by phenol-sulfuric acid method in the extracellular polymers of leaching bacteria.The infrared spectrum was used to detect the structure of the polysaccharide in the extracellular polymers of leaching bacteria.The results show that the optimum conditions are 490 nm for the maximum adsorption wavelength,5 mL of H2SO4 and 5% phenol(mass fraction).The measured value is 12 mg/L for the polysaccharide content in the extracellular polymers of leaching bacteria by phenol-sulfuric acid method in the optimum condition.IR analysis results show that the O—H,C=C,—CH3 and C—H bond exist in the extracellular polymers of leaching bacteria.