The clinical significance of detecting chronic myeloid leukemia bcr/abl fusion gene by real-time quantitative PCR

Objective To investigate the value of diagnosis and prognostic evaluation according to detect the expression of bcr/abl fusion gene in chronic myeloid leukemia by real-time quatitative PCR.Methods To use the real-time PCR to detect the expression of bcr/abl fusion gene of 39 cases chronic myeloid leukemia and 20 cases others hematological diseases.Results Marrow and peripheral blasts showed significant corrilation with the copy of bcr/abl fusion gene.Bcr/abl fusion gene expression level of newly diagnosis chronic myeloid leukemia increased significantly.After the treatment of hydroxycarbamide±interferon,the expression level of the gene decreased markedly.The patients of recurrence or acceleration phase can be found ahead of 2-4 months By the therapy of Glevec or explantation,the most of the fusion gene turned negative.Conclusion The detection of bcr/abl fusion gene is an important way to diagnose chronic myeloid leukemia,and can be a decisive index of treatment and prognostic evaluation.