Objective:To explore the influence of Pine Pollen on anti-oxidative ability in liver of D-gal aging rats. Methods; The sub-acutely aging model rats were made by intraperitoneal injection of D-gal continually. Biochemical methods were used to detect T-AOC, SOD and GSH-PX activities in liver tissue bomogenate of each group. Results- T-AOC, SOD and GSH-PX activities of rats in aging model group significantly decreased compared with normal rats (P0. 01);While the three indexes were remarkably increased after taking Pine Pollen, and there was statistically difference between Pine Pollen and VE (P 0. 01). Conclusions:Pine Pollen could obviously increase the anti-oxidative ability of liver, and has certain anti-aging effects.