Human p53 and Hdm2: Cloning and Construction of Expression Plasmid

The research work reported herein is the cloning and the construction of the expression plasmid of human p53 and Hdm2. The cloning of p53-cDNA and Hdm2-cDNA based on RT-PCR reactions allows for the construction of expression plasmid using the pET-28a (+) transfer vector for the purpose of obtaining recombinant human p53 and Hdm2 in bacteria. The obtained recombinant p53 and Hdm2 proteins will be useful for identifying potent low molecular-weight compounds that block the p53/Hdm2 interaction and permit the activation of p53 and, as a consequence, show anticancer activity.