Daphnetin attenuates the early stage of adipogenesis via suppressing mitotic clonal expansion through Nrf2-mediated redox regulation

abolished the inhibitory effects of daphnetin on MCE and adipogenesis. Consistently, genetic knockdown or pharmacological inhibition of Nrf2 reversed daphnetin-mediated antioxidant signaling and rescued adipogenic progression. Collectively, these findings identify a previously unrecognized Nrf2-ROS-MCE regulatory axis as a key target of daphnetin in adipocyte biology. This study highlights early-stage redox modulation by natural dietary-derived compounds as a promising strategy for preventing obesity and associated metabolic disorders.