The in vitro effects of pulsatile and non-pulsatile blood pumps on human red blood cells were investigated. Group I (Sarns, Inc., non-pulsatile pump) showed significantly less hemolysis (p < 0.05) than Group II (Cobe-Stockert, non-pulsatile roller pump). Group III (Sarns, pulsatile roller pump) and Group IV (Stockert, pulsatile roller pump showed significantly less hemolysis (p < 0.05). Both Sams and Stockert non-pulsatile roller pumps showed significantly (p < .05) less hemolysis at three and four hours of circulation than the Sarns and Stockert pulsatile pumps.