Influence of the chemically reduced graphene oxide interface on the antioxidant multienzyme properties of Prussian blue nanoparticles

Prussian blue nanoparticles (PB NPs) stabilized with surface capping agents as functional mimics of major antioxidant enzymes are attracting attention for use in reactions mediated by reactive oxygen species. However, influence of a stabilizing agent or interface on the enzyme-like properties of the nanomaterial remains unclear. In this study, we investigate the effect of the chemically reduced graphene oxide (rGO) interface on the morphology, stability, and multienzymatic activity of PB NPs. The rGO stabilized PB NPs nanocomposite (PB/rGO) demonstrated different morphology, dynamics of the superoxide scavenging, and lower peroxidase- and catalase-mimicking activities in comparison with the non-stabilized PB NPs. Electrochemical studies showed improved immobilization of PB/rGO on the graphite felt, while the sensitivity of hydrogen peroxide determination was higher for the non-stabilized PB NPs. This study enhances our understanding of the role of the rGO structuring interface in altering the properties of nanomaterials for oxidation protection and nanozyme-based sensing approaches.