The Physiological Adaptation Features of the Poly-Extremophilic Yeast Yarrowia lipolytica W29 During Long-Term Cultivation

Abstract The study of the redox and mitochondria state, as well as some other hallmarks of cell aging of extremophilic yeast Yarrowia lipolytica W29, was performed during long-term cultivation for 120 hours. An increase in the ROS level upon the transition to the stationary growth phase was accompanied by a decrease in the cell respiratory rates, an increase in the alternative oxidase share. Moreover, the activities of superoxide dismutase and catalase increased. During stationary growth phase, the ratio of glutathione/glutathione disulfide increased two to three times. Cytometric studies of the populations in vivo stained with a potentiometric mitotracker showed a decrease in mitochondrial potential as the culture gets old. At the late stationary growth stage, some lysosomal processes in the Y. lipolytica cells were induced that apparently increased the resistance to oxidative stress and allowed the culture keep the survival rate at the level of 80%.