间质细胞
细胞生物学
生物
细胞
骨髓
T细胞
间充质干细胞
免疫学
癌症研究
免疫系统
生物化学
作者
Karin Gustafsson,David T. Scadden
出处
期刊:Springer eBooks
[Springer Nature]
日期:2022-10-19
卷期号:: 191-201
标识
DOI:10.1007/978-1-0716-2679-5_13
摘要
T cells go through most of their maturation in the thymus, and the stromal constituents of the thymus are therefore essential for T cell differentiation. The thymic stroma secretes the factors that recruit and sustain T cell progenitors, and they also partake in the shaping of a functional and tolerant T cell receptor repertoire. The damage incurred to the thymic stromal compartment by bone marrow conditioning regimens as well as by the natural aging process impairs T cell production. Yet little is known of how to prevent or reverse this damage. The development of high-throughput, single-cell analysis technologies has enabled better characterization of thymic stromal cells. This does however require tissue dissociation protocols optimized for stromal cell isolation. In this chapter, we detail the methodology of harvesting thymus stromal cells from human and murine tissue for downstream applications such as flow cytometric analysis and single-cell RNA sequencing.
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