自噬
细胞生物学
受体
先天免疫系统
生物
免疫系统
流式细胞术
吖啶橙
免疫学
生物化学
细胞凋亡
作者
Nisha Singh,Saeid Ghavami,Prashen Chelikani
出处
期刊:Methods in molecular biology
日期:2024-01-01
标识
DOI:10.1007/7651_2024_531
摘要
Microbial dysbiosis is an important trigger in the development of oral diseases. Oral keratinocytes or gingival epithelial cells (GECs) offer protection against various microbial insults. Recent studies suggest that GECs expressed higher level of bitter taste receptor 14 (T2R14) compared to other taste receptors and toll-like receptors and act as innate immune sentinels. Macroautophagy or autophagy is a cellular conserved process involved in the regulation of host innate immune responses against microbial infection. Here, we describe a robust method for evaluation of T2R14-dependent autophagy flux in GECs. Autophagy flux was detected using Western blot analysis in GECs and further was confirmed using Acridine Orange-dependent flow cytometry analysis.
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