灌木岩
壳聚糖
热重分析
生物相容性
材料科学
间充质干细胞
脚手架
傅里叶变换红外光谱
活力测定
组织工程
钙
生物医学工程
化学
化学工程
体外
生物化学
有机化学
工程类
冶金
细胞生物学
生物
医学
作者
José Guilherme Neves,Daniel Navarro da Rocha,Camila Cristine Lopes,Rafael Maza Barbosa,Lorena Fernandes Ferreira,Cecília Buzatto Westin,Ângela Maria Moraes,Bruno Calsa,Milton Santamaria‐Jr,Lourenço Correr-Sobrinho,Américo Bortolazzo Correr
标识
DOI:10.1016/j.ceramint.2022.04.288
摘要
The aim of this study was to synthesize and characterize polymeric porous scaffolds associated with different calcium phosphates (CaP) and Mesenchymal Stem Cells (MSC) for regenerative dentistry application. Chitosan-Xanthan Scaffolds (CX) were associated with 5% of the two CaP types, Hydroxyapatite (HA) and Brushite (BS). For advanced cell therapies, the scaffolds were associated with MSC. The scaffold structures were characterized by X Ray Diffraction (DRX), Fourier Transformed Infrared (FTIR), Thermogravimetric Analysis (TGA), Scanning Electron Microscopy analysis (SEM) and the compressive strength. The in vitro Cytotoxicity was performed and the in vivo Biocompatibility by histomorphometry and inflammatory cells number count. XRD showed the amorphous phase of CX and main peaks of the CaP phases in the HA and BS scaffolds. FTIR showed the amide I and II bands, characteristic of Chitosan, and carboxyl group, characteristic of Xanthan. PO4 bands were found in CaP scaffolds. SEM showed pores and CaP fillers incorporated and adhered to the polymer in the CX-HA, CX-BS, CX-HA + MSC and CX-BS + MSC. Compressive strength and Modulus of Elasticity analysis exhibited higher values for CX-BS scaffolds, followed by CX-HA and CX. All scaffolds showed acceptable cells viability after 24 h and 48 h; however, the CX scaffolds showed higher cell viability in 48 h. CX-BS produced significantly higher inflammatory cells number after 7 and 30 days of implantation. After 60 days of implantation, CX + MSC and CX-HA + MSC showed the lowest inflammatory cells number. The CaP improved the mechanical properties of scaffolds but decreased the cell viability. MSCs improved the inflammatory response after 60 days.
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