NMR spectroscopy for structural elucidation of sulfated polysaccharides from red seaweeds

Some sulfated polysaccharides from red seaweeds are used as hydrocolloids. In addition, it is well known that there are sulfated galactans (carrageenans and agarans) and sulfated mannans, with remarkable biological properties, as antiviral, antitumoral, immunomodulating, antiangiogenic, antioxidant, anticoagulant, and antithrombotic activities, and so on. Knowledge of the detailed structure of the active compound is essential and difficult to acquire. The substitution patterns of the polymer chain, as degree of sulfation and position of sulfate groups, as well as other substituents of the backbone, determine their biological behavior. NMR spectroscopy is a powerful and versatile tool for structural determination. It can be used for elucidation of structures of polysaccharides from new algal sources with novel substitutions or to detect the already known structures from different algal sources, and it could even help to monitor the quality of the active compound on a productive scale. In this review, the available information about NMR spectroscopy of sulfated polysaccharides from red seaweeds is revised and rationalized, to help other researchers working in different fields to study their structures. In addition, considerations about the effects of different structural features, as well as some recording conditions on the chemical shifts of the signals are analyzed.