纳米颗粒
生物高聚物
抗菌剂
化学
溶解
粘附
细菌
纳米技术
大肠杆菌
细菌细胞结构
材料科学
化学工程
生物物理学
生物化学
有机化学
工程类
基因
聚合物
生物
遗传学
作者
S. N. Shevchenko,Markus Burkhardt,Eugene V. Sheval,Ulyana A. Natashina,C. Große,А. Л. Николаев,А. В. Гопин,Ute Neugebauer,A. A. Kudryavtsev,Vladimir Sivakov,Л. А. Осминкина
出处
期刊:Langmuir
[American Chemical Society]
日期:2017-03-02
卷期号:33 (10): 2603-2609
被引量:26
标识
DOI:10.1021/acs.langmuir.6b04303
摘要
In this study, we report a method for the suppression of Escherichia coli (E. coli) vitality by means of therapeutic ultrasound irradiation (USI) using biocompatible silicon nanoparticles as cavitation sensitizers. Silicon nanoparticles without (SiNPs) and with polysaccharide (dextran) coating (DSiNPs) were used. Both types of nanoparticles were nontoxic to Hep 2 cells up to a concentration of 2 mg/mL. The treatment of bacteria with nanoparticles and application of 1 W/cm2 USI resulted in the reduction of their viabilities up to 35 and 72% for SiNPs and DSiNPs, respectively. The higher bacterial viability reduction for DSiNPs as compared with SiNPs can be explained by the fact that the biopolymer shell of the polysaccharide provides a stronger adhesion of nanoparticles to the bacterial surface. Transmission electron microscopy (TEM) studies showed that the bacterial lipid shell was partially perforated after the combined treatment of DSiNPs and USI, which can be explained by the lysis of bacterial membrane due to the cavitation sensitized by the SiNPs. Furthermore, we have shown that 100% inhibition of E. coli bacterial colony growth is possible by coupling the treatments of DSiNPs and USI with an increased intensity of up to 3 W/cm2. The observed results reveal the application of SiNPs as promising antimicrobial agents.
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