自愈水凝胶
透明质酸
甲基丙烯酸酯
细胞外基质
化学
生物物理学
组织工程
光致聚合物
药物输送
细胞包封
材料科学
纳米技术
聚合物
高分子化学
生物医学工程
生物化学
聚合
有机化学
生物
医学
遗传学
作者
Emilie Hachet,Hélène Van Den Berghe,Eric Bayma,Marc R. Block,Rachel Auzély‐Velty
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2012-05-17
卷期号:13 (6): 1818-1827
被引量:115
摘要
Hyaluronic acid (HA) is a natural polysaccharide abundant in biological tissues with excellent potential for constructing synthetic extracellular matrix analogues. In this work, we established a simple and dependable approach to prepare hyaluronic acid-based hydrogels with controlled stiffness and cell recognition properties for use as cell-interactive substrates. This approach relied on a new procedure for the synthesis of methacrylate-modified HA macromers (HA-MA) and, on photorheometry allowing real time monitoring of gelation during photopolymerization. We showed in this way the ability to obtain gels that encompass the range of physiologically relevant elastic moduli while still maintaining the recognition properties of HA by specific cell surface receptors. These hydrogels were prepared from HA macromers having a degree of methacrylation <0.5, which allows to minimize compromising effects on the binding affinity of HA to its cell receptors due to high substitution on the one hand, and to achieve nearly 100% conversion of the methacrylate groups on the other. When the HA hydrogels were immobilized on glass substrates, it was observed that the attachment and the spreading of a variety of mammalian cells rely on CD44 and its coreceptor RHAMM. The attachment and spreading were also shown to be modulated by the elastic properties of the HA matrix. All together, these results highlight the biological potential of these HA hydrogel systems and the needs of controlling their chemical and physical properties for applications in cell culture and tissue engineering.
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