促甲状腺激素受体
互补DNA
生物
分子生物学
促甲状腺激素释放激素受体
肽序列
表达式克隆
cDNA文库
受体
跨膜结构域
分子克隆
甲状腺激素受体
甲状腺
基因
激素受体
生物化学
遗传学
格雷夫斯病
癌症
乳腺癌
作者
Marc Parmentier,Frédérick Libert,Carine Maenhaut,Anne Lefort,Claude Gérard,Jason Perret,Jacqueline Van Sande,Dumont Je,Gilbert Vassart
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1989-12-22
卷期号:246 (4937): 1620-1622
被引量:488
标识
DOI:10.1126/science.2556796
摘要
The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors. Out of 11 new putative receptor clones obtained from genomic DNA, one had sequence characteristics different from all the others. Although this clone did not hybridize to thyroid transcripts, screening of a dog thyroid complementary DNA (cDNA) library at moderate stringency identified a cDNA encoding a 4.9-kilobase thyroid-specific transcript. The polypeptide encoded by this thyroid-specific transcript consisted of a 398-amino acid residue amino-terminal segment, constituting a putative extracellular domain, connected to a 346-residue carboxyl-terminal domain that contained seven putative transmembrane segments. Expression of the cDNA conferred TSH responsiveness to Xenopus oocytes and Y1 cells and a TSH binding phenotype to COS cells. The TSHR and the receptor for luteinizing hormone-choriogonadotropin constitute a subfamily of G protein-coupled receptors with distinct sequence characteristics.
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