化学
超氧化物歧化酶
谷胱甘肽过氧化物酶
阴沟肠杆菌
抗氧化剂
乙醇沉淀
生物化学
半乳糖
多糖
甘露糖
阿布茨
乳糖
微生物学
DPPH
生物
肠杆菌科
大肠杆菌
基因
作者
Mingliang Jin,Youming Wang,Chunlan Xu,Zeqing Lu,Ming Huang,Yizhen Wang
标识
DOI:10.1016/j.carbpol.2010.03.020
摘要
A water-soluble extracellular polysaccharide (EPS-1) was isolated from the submerged culture broth of Enterobacter cloacae Z0206 through fermentation, ethanol precipitation, anion-exchange and gel-permeation chromatography. Its structural analysis showed that the average molecular weight of EPS-1 was 23,928 Da, and it was composed of glucose, mannose and galactose with a molar ratio of 6.860:1.180:0.455. It was hypothesized that EPS-1 belongs to the α-type heteropolysaccharide with pyran group. Bioactivity tests in vivo showed that the administration of EPS-1 to cyclophosphamide (CP)-exposed animals resulted in significant increase and recovery of B lymphocyte proliferation, tumor necrosis factor α (TNF-α) production and activities of antioxidant enzymes (superoxide dismutase (SOD) and glutathione peroxidase (GPx)). It is suggested that EPS-1 could provide protection against CP-induced immunosuppression and oxidative damage in mice model, and it may act as a potent immunomodulatory and antioxidant agent.
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