MMP1型
时间1
阿达姆斯
软骨
基质金属蛋白酶
MMP2型
MMP3型
基因表达
金属蛋白酶
基因
骨关节炎
基因表达谱
软骨细胞
聚蛋白多糖酶
MMP9公司
分子生物学
病理
医学
生物
内科学
关节软骨
遗传学
下调和上调
解剖
血栓反应素
替代医学
作者
Lara Kevorkian,David A. Young,Clare Darrah,Simon Donell,Lee Shepstone,Sarah Porter,Sarah Brockbank,Dylan R. Edwards,Andrew E. Parker,Ian M. Clark
摘要
Abstract Objective To profile the expression of all known members of the matrix metalloproteinase (MMP), ADAMTS, and tissue inhibitor of metalloproteinases (TIMP) gene families in normal cartilage and cartilage from patients with osteoarthritis (OA). Methods Human cartilage was obtained from femoral heads at joint replacement for OA or following fracture to the femoral neck. Total RNA was purified, and gene expression was assayed using quantitative real‐time polymerase chain reaction. Results Several members of the above gene families were regulated in OA. Genes that showed increased expression in OA were MMP13 , MMP28 , and ADAMTS16 (all at P < 0.001), MMP9 , MMP16 , ADAMTS2 , and ADAMTS14 (all at P < 0.01), and MMP2 , TIMP3 , and ADAMTS12 (all at P < 0.05). Genes with decreased expression in OA were MMP1 , MMP3 , and ADAMTS1 (all at P < 0.001), MMP10 , TIMP1 , and ADAMTS9 (all at P < 0.01), and TIMP4 , ADAMTS5 , and ADAMTS15 (all at P < 0.05). Correlation analysis revealed that groups of genes across the gene families were coexpressed in cartilage. Conclusion This is the first comprehensive expression profile of all known MMP, ADAMTS, and TIMP genes in cartilage. Elucidation of patterns of expression provides a foundation with which to understand mechanisms of gene regulation in OA and potentially to refine the specificity of antiproteolytic therapies.
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