单核吞噬细胞系统
生物
吞噬细胞
外周血单个核细胞
单核细胞
体外
细胞因子
巨噬细胞
肿瘤坏死因子α
免疫学
抗原
细胞生物学
分子生物学
内分泌学
内科学
吞噬作用
生物化学
医学
作者
Marina Kreutz,Reinhard Andreesen
出处
期刊:Blood
[American Society of Hematology]
日期:1990-12-15
卷期号:76 (12): 2457-2461
被引量:118
标识
DOI:10.1182/blood.v76.12.2457.2457
摘要
Abstract Cells of the mononuclear phagocyte system arise from circulating blood monocytes (MO) that undergo further maturation on leaving the vasculature and migration into the various tissues and body cavities. This terminal differentiation step is also observed in vitro when blood MO are cultured in the presence of serum. Yet, the inducing signals present in serum are not defined. We have established primary cultures from elutriation-purified blood MO and found that the active metabolite of vitamin D3 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) could induce maturation of MO to macrophages (MAC) in the absence of any serum proteins. Cells were cultured for 7 days with AB-group serum or 1,25(OH)2D3, respectively, and MO maturation analyzed by morphology, functional activity, and the expression of lineage-restricted maturation-associated antigens (MAX.1, MAX.3). At an optimal concentration of 10(-8) mol/L, 1,25(OH)2D3 promoted the development of fully differentiated MAC whose phenotype and functional competence in terms of cytokine release (tumor necrosis factor alpha, interleukin-6, fibronectin, and lysozyme) was comparable with MAC grown in serum. In conclusion, our data may add to the immunoregulatory potential of 1,25(OH)2D3, which may play an essential role in the ontogeny of the mononuclear phagocyte system.
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