生物
基因
微阵列
互补DNA
结直肠癌
癌症研究
基因表达
分子生物学
癌症
转移
微阵列分析技术
DNA微阵列
基因表达谱
遗传学
作者
Motoyuki Otsuka,Masaki Kato,Tsutomu Yoshikawa,Hua Chen,Eric J. Brown,Yasuhiko Masuho,Masao Omata,Naohiko Seki
标识
DOI:10.1006/bbrc.2001.6047
摘要
To identify molecular alterations in the progression of colorectal carcinoma, we analyzed gene expression profiles of colon cancer cell lines derived from primary and metastatic tumors from a single patient. Of 2280 cDNAs investigated using our in-house microarray, the expression of 6 genes (tumor-associated antigen L6, L-plastin, the human homologue of yeast ribosomal protein S28, the B-cell translocation gene, mitochondrial aspartate-aminotransferase, and HLA-A) increased, while that of 2 genes (keratin 5 and phosphoglucomutase) decreased in metastatic-tumor-derived cells compared with primary-tumor-derived cells. Of these genes, we assessed the L-plastin gene, an actin-bundling protein, at the protein level using a tissue microarray consisting of 58 clinically stratified colorectal cancer specimens. Consistent with our microarray results, the expression of L-plastin was significantly correlated with the progression of cancer staging. Therefore, our results suggest that the L-plastin gene is a potential metastatic marker. In addition, combining cDNA microarrays and tissue arrays, as shown here, is thought to facilitate the rapid characterization of candidate biomarkers.
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