代谢组学
轨道轨道
亲水作用色谱法
色谱法
化学
质谱法
样品制备
液相色谱-质谱法
工作流程
协议(科学)
计算机科学
高效液相色谱法
数据库
医学
病理
替代医学
作者
Amanda Souza,Gary J. Patti
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 357-382
被引量:7
标识
DOI:10.1007/978-1-0716-1266-8_27
摘要
Untargeted metabolomics has rapidly become a profiling method of choice in many areas of research, including mitochondrial biology. Most commonly, untargeted metabolomics is performed with liquid chromatography/mass spectrometry because it enables measurement of a relatively wide range of physiochemically diverse molecules. Specifically, to assess energy pathways that are associated with mitochondrial metabolism, hydrophilic interaction liquid chromatography (HILIC) is often applied before analysis with a high-resolution accurate mass instrument. The workflow produces large, complex data files that are impractical to analyze manually. Here, we present a protocol to perform untargeted metabolomics on biofluids such as plasma, urine, and cerebral spinal fluid with a HILIC separation and an Orbitrap mass spectrometer. Our protocol describes each step of the analysis in detail, from preparation of solvents for chromatography to selecting parameters during data processing.
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