蛋白质酪氨酸磷酸酶
酪氨酸
原癌基因酪氨酸蛋白激酶Src
SH2域
脱磷
肽
生物化学
化学
残留物(化学)
苯丙氨酸
肽序列
磷酸化
磷酸酶
氨基酸
分子生物学
生物
基因
作者
Kazuya Tsumagari,Tomoya Niinae,Akira Otaka,Yasushi Ishihama
出处
期刊:Proteomics
[Wiley]
日期:2021-12-21
卷期号:22 (4)
被引量:1
标识
DOI:10.1002/pmic.202100144
摘要
We developed peptide probes containing a non-hydrolyzable phosphotyrosine mimetic, 4-[difluoro(phosphono)methyl]-L-phenylalanine (F2 Pmp) for the enrichment of protein tyrosine phosphatases (PTPs). We found that different F2 Pmp probes can enrich different PTPs, depending on the probe sequence. Furthermore, proteins containing a Src homology 2 (SH2) domain were enriched together. Importantly, probes containing phosphotyrosine instead of F2 Pmp failed to enrich PTPs due to dephosphorylation during the pulldown step. This enrichment approach using peptides containing F2 Pmp could be a generic tool for tyrosine phosphatome analysis without the use of antibodies.
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