化学
膜联蛋白
细胞凋亡
细胞毒性
细胞毒性T细胞
免疫印迹
药理学
传统医学
蛋白激酶B
癌症研究
生物
生物化学
医学
体外
基因
作者
Haohao Wang,Wenliu Yu,Danfeng Zhang,Yan Zhao,Chen Chen,Hongyan Zhu,Enbo Cai,Zhaowei Yan
标识
DOI:10.1080/14786419.2020.1844698
摘要
A rich of 3,4-seco-lupane triterpenoids (3,4-SLT), including chiisanoside (CSS), divaroside (DVS), sessiloside-A1 (SSA), chiisanogenin (CSG), sessiligenin (SSG), were isolated from the ethanol extract of the leaves of Eleutherococcus sessiliflorus (LES). The present study was performed to explore the cytotoxic and anti-tumor effects of the isolated five ones, as well as potential molecular mechanisms. The results of a CCK-8 assay demonstrated that these 3,4-SLT can inhibit the growth of HepG2 cells, and SSG showed the most significant cytotoxicity. Hoechst 33258 fluorescence staining and Annexin V-FITC/PI staining indicated that 3,4-SLT in LES can induce HepG2 cell apoptosis effectively. The AutoDock Vina program was used to simulate molecular docking of drugs and targets to discuss possible pharmacological mechanisms. Besides, western blot and qRT-PCR results indicated that SSG can inhibit PI3K/AKT signaling pathway through controlling multi-targets. This study suggested that 3,4-SLT might become a new research hotspot for antineoplastic drugs.
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