去甲基化
DNA去甲基化
太赫兹辐射
DNA甲基化
甲基化
脱甲基剂
辐照
材料科学
化学
太赫兹光谱与技术
癌症研究
生物物理学
光电子学
光学
黑色素瘤
光化学
作者
Hwayeong Cheon,Hee-Jong Yang,Moran Choi,Joo‐Hiuk Son
标识
DOI:10.1364/boe.10.004931
摘要
Terahertz (THz) demethylation is a photomedical technique applied to dissociate methyl-DNA bonds and reduce global DNA methylation using resonant THz radiation. We evaluated the performance of THz demethylation and investigated the DNA damage caused by THz irradiation. The demethylation rate in M-293T DNA increased linearly with the irradiation power up to 48%. The degree of demethylation increased with exposure to THz radiation, saturating after 10 min. Although THz demethylation occurred globally, most of the demethylation occurred within the partial genes in the CpG islands. Subsequently, we performed THz demethylation of melanoma cells. The degree of methylation in the melanoma cell pellets decreased by approximately 10-15%, inducing ∼5-8 abasic sites per 105 bp; this was considerably less than the damaged DNA irradiated by the high-power infrared laser beam used for generating THz pulses. These results provide initial data for THz demethylation and demonstrate the applicability of this technique in advanced cancer cell research. THz demethylation has the potential to develop into a therapeutic procedure for cancer, similar to that involving chemical demethylating agents.
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