代谢工程
大肠杆菌
发酵
工业与生产工程
倍他林
化学
组氨酸
食品科学
生物
生物化学
氨基酸
颜料
酶
基因
有机化学
工程类
电气工程
作者
Yanan Hou,Xue Li,Shilin Li,Xue Zhang,Yu Sili,Guang‐Rong Zhao
标识
DOI:10.1021/acs.jafc.0c02949
摘要
Betalains are emerging natural pigments with high tinctorial strength and stability, physiological activities, and fluorescent properties for potential application in food, cosmetic, and pharmaceutical industries. Betalains including yellow betaxanthins and red betacyanins are mainly restricted in the Caryophyllales plants. To expand the availability of individual betaxanthins, here, we constructed an Escherichia coli BTA6 for de novo biosynthesis of betalamic acid. Using this strain as a monoculture platform, 14 yellow and 2 red betaxanthins were produced by feeding amino acids and amines. Furthermore, we constructed an l-histidine overproducing strain using chromosome engineering to deattenuate regulation and established a coculture system. After optimization of the initial inoculation ratios and fermentation conditions, the compatible and robust coculture system produced 287.69 mg/L of histidine-betaxanthin. This is the first report on de novo production of betaxanthins in engineered E. coli using glucose as a carbon source. Our work highlights the feasibility of microbial cell factories to produce individual betalains.
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