肠易激综合征
医学
微生物群
逻辑回归
粪便
内科学
胃肠病学
机器学习
生物信息学
微生物学
生物
计算机科学
作者
Hirokazu Fukui,Akifumi Nishida,Satoshi Matsuda,Fumitaka Kira,Satoshi Watanabe,Minoru Kuriyama,Kazuhiko Kawakami,Yoshiko Aikawa,Noritaka Oda,Kenichiro Arai,Atsushi Matsunaga,Masahiko Nonaka,Katsuhiko Nakai,Wahei Shinmura,Masao Matsumoto,Shinji Morishita,Aya Takeda,Hiroto Miwa
摘要
Irritable bowel syndrome (IBS) is diagnosed by subjective clinical symptoms. We aimed to establish an objective IBS prediction model based on gut microbiome analyses employing machine learning. We collected fecal samples and clinical data from 85 adult patients who met the Rome III criteria for IBS, as well as from 26 healthy controls. The fecal gut microbiome profiles were analyzed by 16S ribosomal RNA sequencing, and the determination of short-chain fatty acids was performed by gas chromatography–mass spectrometry. The IBS prediction model based on gut microbiome data after machine learning was validated for its consistency for clinical diagnosis. The fecal microbiome alpha-diversity indices were significantly smaller in the IBS group than in the healthy controls. The amount of propionic acid and the difference between butyric acid and valerate were significantly higher in the IBS group than in the healthy controls (p < 0.05). Using LASSO logistic regression, we extracted a featured group of bacteria to distinguish IBS patients from healthy controls. Using the data for these featured bacteria, we established a prediction model for identifying IBS patients by machine learning (sensitivity >80%; specificity >90%). Gut microbiome analysis using machine learning is useful for identifying patients with IBS.
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