Streptomyces biotransformation of ferulic acid to vanillic acid

Streptomyces2036 was screened for its ability to transform ferulic acid to vanillic acid.The optimal medium was found to contain glucose(5 g/L) and yeast extract(5 g/L).Cultures in 250 mL flasks containing 50 mL medium were brought to pH 5.0 and incubated by shaking at 150 r/min at 30 ℃.Under these conditions,Streptomyces2036 produced vanillic acid when ferulic acid was added 16 h after inoculation.After a further 12 h,1 g/L ferulic acid was transformed to 0.261 g/L vanillic acid with a molar yield of 30.2%.It was shown that intracellular enzyme was mainly responsible for catalyzing the transformation of ferulic acid to vanillic acid.