抗原
结核分枝杆菌
肺结核
生物
结核病疫苗
蛋白质组学
病毒学
免疫学
dna疫苗
反向疫苗学
表位
卡介苗
计算生物学
接种疫苗
医学
免疫
遗传学
基因
病理
作者
Shingar Sharma,Raju S. Rajmani,Arun Kumar,Ashima Bhaskar,Amit Singh,Venkatasamy Manivel,Anil K. Tyagi,Kanury V. S. Rao
出处
期刊:PubMed
日期:2015-07-01
卷期号:53 (7): 429-39
被引量:2
摘要
Unsatisfactory performance of the existing BCG vaccines, especially against the adult pulmonary disease, has urged the need for an effective vaccine against tuberculosis (TB). In this study, we employed differential proteomics to obtain a list of antigens as potential vaccine candidates. Bacterial epitopes being presented at early stages on MHC class I and class II molecules of macrophages infected with Mycobacterium tuberculosis (M. tb) were identified using iTRAQ labelling and reverse phase LC-MS/MS. The putative vaccine candidates, thus identified, were tested as plasmid DNA vaccines in mice to ascertain their protective efficacy against the aerosolized M. tb challenge, based on their ability to reduce the bacterial load in the lungs of infected mice. Here, we observed that 4 out of the 17 selected antigens imparted significant protection against the challenge of M. tb. The four shortlisted antigens were further assessed in a more stringent guinea pig model, where too, they demonstrated.significant protection. It concludes that combining a proteomics approach with the in vivo assessment of vaccine candidates in animal models can be valuable in identifying new potential candidates to expand the antigenic repertoire for novel vaccines against TB.
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