SCREENING HIGH-PRODUCTION LACCASE OF PLEUROTUS OSTREATUS AND DEGRADATION OF AFB_1 BY PLEUROTUS OSTREATUS LACCASE

Two high laccase-production strains Pleurotus ostreatus P1 and P2 were selected by selecting agar plates and liquid culture.MSM medium was the most suitable liquid medium for laccase-production.The best strain for laccase-producing and AFB1-degradating was Pleurotus ostreatus strain P1.The laccase activity could be 769.44U/L when it was cultured 10 days.1000ng AFB1 could be degradated to 222.62ng by 790μl crude enzyme solution and the degradation rate of AFB1 was 77.74%.There were positive corvelootions between AFB1-degradating and laccase actitity.