Inhibition effects of metformin on calcification in rat vascular smooth muscle cells induced by advanced glycation end products

Objective: To investigate the inhibition effects of metformin on calcification in vascular smooth muscle cells(VSMCs) induced by advanced glycation end products(AGEs) on cell level.Methods: VSMCs(A7R5) were used in vitro experiments.The cells were divided into control group,BSA group,AGE-BSA group,common interfere with metformin and AGE-BSA group.VSMCs(A7R5) were incubated with 200 mg·L-1 AGE-BSA and different concentrations of metformin(10-4,5×10-5,10-5 mol·L-1) in DMEM with 10 mmol·L-1β-glycerophosphate.The effects of various concentrations of metformin on VSMCs calcification were observed.Calcium deposition was detected by automatic biochemical analyzer.The activity of ALP was detected by the method of disodium phenyl phosphate.The mRNA and protein expression of BMP-2 were detected by RT-PCR and Western blotting,respectively.Results: Compared with control group,the calcium deposition and ALP activity of AGE-BSA group were increased obviously,and the expression of cell BMP-2 mRNA and protein were all elevated.The expression of these indicators induced by AGE-BSA was declined in different concentration of metformin group.Conclusion: AGE-BSA could promote calcification of VSMCs,and metformin can inhibit VSCMs calcification induced by AGE-BSA in dose-dependently.