Stallion Semen Preservation Selection of Extenders and Effection Observation

The stallion semen preservation extenders and dosage forms in different temperatures were selected in this experiment. The results showed that: (1) The sperm motility in 2.5%Gly extender, 7%Glu extender were higher significantly than that in 5%Gly extender, 0.9%Nacl extender(P0.05), and the survival time was extreme significant(P0.01) in room temperature,. (2)In low- temperature, the sperm motility in 10%Milk powder extender, 5%Egg yolk extender were higher significantly than that in 0.8%Egg yolk extender(P0.05) and extreme significantly than that in 7%Glu extender(P0.01), and the survival time was longer extreme significant than that in 0.8%Egg yolk extender, 7%Glu extender(P0.01). (3)In cryopreservation, the pellet frozen semen motility in 11%Lac extender, 10%Suc extender, 4%Fructose extender were extreme significantly than that in 0.1%EDTA extender, 3%Glu extender, 1.25%Tris extender(P0.01). The straw semen motility were higher significantly than that in 3%Glu extender, 1.25%Tris extender(P0.05) and 0.1%EDTA extender extreme significantly(P0.01). Compared with the straw semen, the frozen-thawing motility and recovery of the pellet semen were significantly different(P0.05). Acorosomal intrgrity, in 10%Suc extender, 4%Fructose extender were higher extreme significantly than that in 11%Lac extender(P0.01), and higher significantly than that in 11%Lac in abnormality(P0.05). There was no difference between frozen pellet semen and straw semen in frozen-thawing motility(P0.05).