Study on gold immunochromatography assay for rapid detection of fluoroquinolones

To establish a rapid assay for detecting fluoroquinolones in milk,gold immunochromatography assay was studied,which was used with a readout instrument? of colloidal gold test paper card together.The limit of detection(enrofloxacin,sarafloxaxcin,difloxacin,ofloxacin,norfloxacin,ciprofloxacin,pefloxacin,flumequine,danofloxacin) is 20 μg/L,and(enoxacin,oxolinic acid) is 40 μg/L.And we can obtain the results in 10 minutes.The assay gives no false positive and false negative results.The method is accurate,simple,reliable and convenient to rapidly detect fluoroquinolones of a lot of samples on the spot.