Analysis of Cerebral Tissue Damage and Expression of C9 and NO at Different Time of Rats after Hypertensive Intracerebral Hemorrhage

Objective To investigate cerebral tissue damage degree and the expression of complement C9 and NO in rats at different time after hypertensive intracerebral hemorrhage( ICH). Methods Rat ICH model was established by Collagenase infusion. 96 rats were divided into 3 groups,32 rats in each group. Each group was randomly subdivided into sham group and model group. Brain water content and the levels of nitric oxide( NO) and C9 were determined at 2h,12h,24h and 72h after ICH. Results Brain water contents and expression of C9 in model group were increased remarkably 24 to 72 hours after operations,while the content of NO was decreased as compared to sham group. Conclusion Cerebral tissue damage degree in acute intracerebral hemorrhage was associated with content of NO,expression of C9 in rats 'cerebral tissues. It's important to improve brain circulation and alleviate inflammatory response.