Comparison of indirect indirect immunofluorescence and immunobloting for detecting ACA

Objective To investigate clinical application value of anti-centromere antibody(ACA) detected by indirect indirect immunofluorescence(IIF)and immunobloting(IB) methods.Methods Serum collected from76 patients with difierent autoimmune diseases and 50 healthy volunteers were detected by IIF and IB.Results There was no significant difference between IIF and IB methods for ACA(P0.05).IIF ACA positively correlated with the number of immunofixed CENP-B bands(r=0.871,P0.01).The positive coincidence rates for IIF/IB and IB/IIF were 91.9%(68/74) and 97.1%(68/70),respectively.While the negative coincidence rate was 100.0%(50/50).Conclusion The measure effects of IIF and IB for ACA are almost equivalent.But IIF is a simple,rapid and accurate method for detecting ACA,which can be applied in clinical routine test.