Studies on the neuro-cell apoptosis and expression of Bcl-2 artment/Bax and caspase-3 after traumatic cerebral contusion in human

Objective To study on whether the apoptosis existed and the change of gene expression related to apoptosis by investigation of apoptosis of the cerebral contusion and expression of Bcl-2/Bax and caspase-3 related to apoptosis in human.Method We researched that apoptosis and gene expression of Bcl-2/Bax and caspase-3in brain tissues by FCM and immunohistochemical.Results Analysis showed that apoptosis of neuro-cell was related to patient's injury serious level and injury time(P0 05),but was not related to prognosis.The positive expression of Bcl-2 was related to prognosis(P0 05),but was not related to sex?age?GCS and injury time.The caspase-3 was highly correlated with the apoptosis,which showed the expression of caspase-3 was an indicator of apoptosis degree.Conclusions Apoptosis occurred in human brain after actue injury which fulfilled a function in pathological transformation of brain tissues. The expression of Bcl-2 was related to prognosis. The expression of caspase-3 could reflect the levels of apoptosis.