Determination of γ-Amino Butyric Acid in Lactobacillus brevis Fermentation Liquid by HPLC

High performance liquid chromatography with o-phthaldialdelhyde pre-colomn derivatization was used to determine the content of γ-amino butyric acid (GABA) in Lactobacillus brevis. After pre-colomn derivatization of concentrated fermentation supernatant, separation of GABA was carried out on Nova2pakC18 column with the gradient elution of 50 mmol/L acetate sodium (pH 6.8), methanol and THF (solution A 82:17:1; solution B 22:77:1, V/V). The results of accuracy and recovery rate showed that this menthod can be used to detect trace content of GABA.