Pseudorabies virus Isolated and Indentified of Xinjiang

One Pseudorabies(Aujeszky's disease) virus isolated from infected domestic pigs originating from Wujiaqu shihezi of ×injiang.The organ samples were filtered then drained monolayers of BHK-21 cells,the typical cytopathic effect(cpe) were observed. The virus appeared typical Pseudorabies virus form:diameter was about 100 nm,circle with peplos and peplomer .The virus were neutralized with standard positive porcine sera using serum neutralization test(SNT). TCID_(50) of virus was 10-10.87/0.1mL. Rabbits showed typical syndrome of PR when they were inoculated with cells virus. PCR technique was applied for the detection PRV in cells and Amplified a fragment with desired size of 217 bp.According with virus characterisation from infected pigs,the virus was indentified as PRV.