Optimization of Cell Suspension Culture Conditions of Lisgusticum Chuanxiong at High Biomass Yield

To set up cell suspension culture system of Lisgusticum chuanxiong and optimize cell culture growth conditions,using MS as the basic medium,we study the impact of hormone concentrations, inoculum and other factors on the growth of suspension cells of Lisgusticum chuanxiong. The experimental results show that,when the synchronized suspension cells subculture of Lisgusticum chuanxiong grows about 16 days to the logarithmic growth medium-late period,according to 1∶ 4( V / V) suspension which includes fresh culture liquid,MS + 6- BA 0. 5 mg·L- 1+ 2,4- D 2 mg·L- 1as optimal culture medium,higher yields of suspension cells of Lisgusticum chuanxiong can be obtained,whose wet weight and dry weight can reach 182. 6 g·L- 1and 39. 2 g·L- 1respectively.